Publications 2021~ Sun W, Wang M, Zhao J, Zhao S, Zhu W, Wu X, Li F, Liu W, Wang Z, Gao M, Zhang Y, Xu J, Zhang M, Wang Q, Wen ZL, Shen J, Zhang W, Huang Z. (2023) Sulindac selectively induces autophagic apoptosis of GABAergic neurons and alters motor behaviour in zebrafish. Nature Communications 14 (1):5351. 37453064Zhou QX#, Zhao CL#, Yang ZY, Qu R, Li YB, Fan YN, Tang JL, Xie T, Wen ZL (2023) Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish. Cell Reports 42:112793. Yu T#, Kuang HY#, Huang Y, Wu XH, Wang JZ, Wen ZL (2023) Cell competition for neuron-derived trophic factor controls the turnover and lifespan of microglia. Science Advances 9: eadf9790. Zhao CL, Li YB, Tang JL, Zhou QX, Lin X, Wen ZL (2023) Metaphocytes are IL-22BP-producing cells regulated by ETS transcription factor Spic and essential for zebrafish barrier immunity. Cell Reports 42(5):112483. Dai YM#, Wu ST#, Xue RT, Luo XF, Wen ZL*, Xu J*. (2022) Csf1rb regulates definitive hematopoiesis in zebrafish. Development 149: dev200534. (*Co-corresponding author) Lou L#, Yu T#, Dai YM, Zhao SZ, Feng SC, Xu J, Wen ZL. (2022) Mafba and Mafbb regulate microglial colonization of zebrafish brain via controlling chemotaxis receptor expression. Proceedings of the National Academy of Sciences USA 119: e2203273119. (# equal contribution) 129:825-839. Zhao SZ#, Zhang A#, Zhu H, and Wen ZL. (2022) The ETS transcription factor Spi2 regulates hematopoietic cell development in zebrafish. Development 149: dev200881. (# equal contribution) Zhao SZ#, Feng SC#, Tian Y#, Wen ZL. (2022) Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage. Proceedings of the National Academy of Sciences USA. 2022 Mar 29;119(13):e2119051119. Li H, Nam YH, Huo R, Fu WL, Jiang BB, Zhou QX, Song D, Yang YX, Jiao YM, Weng JC, Yan ZH, Di L, Li J, Wang J, Xu HY, Wang S, Zhao JZ, Wen ZL, Wang JG, Cao Y. (2021) De Novo germline and somatic variants convergently promote endothelial-to-mesenchymal transition in simplex brain arteriovenous malformation. Circulation Research 129:825-839. Zhao Y, Wu M, Li J, Meng P, Chen JK, Huang ZB, Xu J, Wen ZL, Zhang WQ, Zhang YY. (2021) The spliceosome factor Sart3 regulates hematopoietic stem/progenitor cell development in zebrafish through the p53 pathway. Cell Death & Disease 12:1-12. Chen JK, Li G, Lian J, Ma N, Huang ZB, Li J, Wen ZL, Zhang WQ, Zhang YY. (2021) Slc20a1b is essential for hematopoietic stem/progenitor cell expansion in zebrafish. Science China Life Sciences 1-16. 2016~2020 Wu ST#, Nguyen TML#, Pan HR, Hassan S, Dai YM, Xu J, Wen ZL (2020) Two phenotypically and functionally distinct microglial populations in adult zebrafish. Science Advances 6:eabd1160. (# equal contribution) Lin X#, Zhou QX#, Lin GZ, Zhao CL, Wen ZL (2020) Endoderm-derived myeloid-like metaphocytes in zebrafish gill mediate soluble antigen-induced immunity. Cell Reports 33:108227. (# equal contribution) Yu T, Kuang HY, Chen JH, Lin X, Wu Y, Chen KY, Zhang MJ, Zhang WQ*, and Wen ZL* (2020) Tripartite-motif family protein 35-28 regulates microglia development by preventing necrotic death of microglial precursors in zebrafish. The Journal of Biological Chemistry 295:8846-8856. (*Co-corresponding author) He SC, Xu J, Qu JY*, Wen ZL* (2020) Lightening the way of hematopoiesis: infrared laser-mediated lineage tracing with high spatial-temporal resolution. Experimental Hematology 85:3-7. (Invited review) (*Co-corresponding author) He SC#, Tian T#, Feng SC#, Wu Y, Shen XW, Chen K, He YZ, Sun QQ, Li XS, Xu J*, Wen ZL*, and Qu JNY* (2020) In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy. eLife 9:e52024. (*co-corresponding author) (#equal contribution) Wang TN#, Yan B#*, Lou L., Lin X, Yu T, Wu ST, Lu Q, Liu W, Huang ZB, Zhang MJ, Zhang WQ*, and Wen ZL*. (2019) Nlrc3-like is required for microglia maintenance in zebrafish. Journal of Genetics and Genomics 46:291-299. (*co-corresponding author) (#equal contribution) Lin X, Zhou QX, Zhao CL, Lin GZ, Xu J, and Wen ZL. (2019) An ectoderm-derived myeloid-like cell population functions as antigen transporters for Langerhans cells in zebrafish epidermis. Developmental Cell 49:605-617. Jin W#, Dai YM#, Li FN, Zhu L, Huang ZB, Liu L, Li JC, Zhang MJ, Du JL, Zhang WQ*, and Wen ZL*. (2019) Dysregulation of microglial function contributes to neuronal impairment in Mcoln1a-deficient zebrafish. iScience 13:391-401. Wu ST, Xue RT, Hassan SL, Nguyen TML, Wang TN, Pan HR, Liu QF, Xu J, Zhang WQ, and Wen ZL. (2018) Il34-Csf1r pathway regulates the migration and colonization of microglial precursors. Developmental Cell 46:552-563. He SC, Chen JH, Jiang YY, Wu Y, Zhu L, Jin W, Zhao CL, Yu T, Wang TN, Wu ST, Lin X, Qu JN, Wen ZL, Zhang WQ* and Xu J*. (2018) Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells. eLife 7:e36131. Tian Y, Xu J, Feng SC, He SC, Zhao SZ, Zhu L, Jin W, Dai YM, Luo LF, Qu JY, and Wen ZL. (2017) The first wave of T lymphopoiesis in zebrafish arises from aorta endothelium independent of hematopoietic stem cells. The Journal of Experimental Medicine 214:3347-3360. Yu T, Guo WL, Tian Y, Xu J, Chen JH, Li L, and Wen ZL. (2017) Distinct regulatory networks control the development of macrophages of different origins in zebrafish. Blood 129:509-519. Liu W, Wu M, Huang ZB, Lian JW, Chen JH, Wang TN, Leung AYH, Liao YL, Zhang ZH, Yen KY, Lin S, Zon LI, Wen ZL, Zhang YY, and Zhang WQ. (2017) c-Myb hyperactivity leads to myeloid and lymphoid malignancies in zebrafish. Leukemia 31:222-233. Dai YM, Zhu L, Huang ZB, Zhou MY, Jin W, Liu W, Xu MC, Yu T, Zhang YY, Wen ZL*, Liao WJ*, and Zhang WQ*. (2016) Cebpα is essential for the embryonic myeloid progenitor and neutrophil maintenance in zebrafish. Journal of Genetics and Genomics 43:593-600 (*Co-corresponding author). Xu J, Wang TN, Wu Y, Jin W, and Wen ZL. (2016) Microglia colonization of developing zebrafish midbrain is promoted by apoptotic neuron and lysophosphatidylcholine. Developmental Cell 38:214-222. Jin H, Huang ZB, Chi YL, Wu M, Zhao LF, Zhou RY, Xu J, Zhen FH, Lan YH, Li L, Zhang WQ, Wen ZL*, and Zhang YY*. (2016) c-Myb acts in parallel and cooperatively with Cebp1 to regulate neutrophil maturation in zebrafish. Blood 128:415-426. (Co-corresponding author) Wei YL, Xu J, Zhang WQ, Wen ZL, and Liu F. (2016) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development 143:2103-2110. 2011~2015 Xu J, Zhu L, He SC, Wu Y, Jin W, Yu T, Qu JY, and Wen ZL. (2015) Temporal-spatial resolution fate mapping reveals distinct origins for embryonic and adult microglia in zebrafish. Developmental Cell 34:632–641. Shi XG, He BL, Ma CH, Guo YH, Man CH, Ekker SC, Zhang WQ, Zhang YY, Wen ZL, and Leung YH. (2015) Functions of idh1 and its mutation in the regulation of developmental hematopoiesis in zebrafish. Blood 125:2974-2984 Yuan H, Zhang T, Liu X, Deng M, Zhang W, Wen ZL, Chen S, Chen Z, de The H, Zhou J, and Zhu J. (2015) Sumoylation of CCAAT/enhancer-binding protein α is implicated in hematopoietic stem/progenitor cell development through regulating runx1 in zebrafish. Scientific Reports 5:9011 Zeng Y, Yan B, Xu J, Sun QQ, He SC, Jiang J, Wen ZL, and Qu JY. (2014) In vivo nonlinear optical imaging of immune responses: tissue injury and infection. Biophysical Journal 107: 2436-2443. Yan B, Han PD, Pan LF, Lu W, Xiong JW, Zhang MJ, Zhang WQ, Li L, and Wen ZL. (2014) Il-1β and reactive oxygen species differentially regulate neutrophil directional migration and Basal random motility in a zebrafish injury-induced inflammation model. The Journal of Immunology 192:5998-6008. Zeng Y, Yan B, Sun QQ, He SC, Jiang J, Wen ZL, and Qu JY. (2014) In vivo micro-vascular imaging and flow cytometry in zebrafish using two-photon excited endogenous fluorescence. Biomedical Optics Express 5: 653-663. Zhen FH, Lan YH, Yan B, Zhang WQ, and Wen ZL. (2013) Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms. Development 140:3977-3985. (F1000 recommended) Sun JP, Liu W, Li L, Chen JH, Wu M, Zhang YY, Leung AYH, Zhang WQ*, Wen ZL*, and Liao WJ*. (2013) Suppression of Pu.1 function results in expanded myelopoiesis in zebrafish. Leukemia 27:1913-1917. (Co-corresponding author) Zeng Y, Yan B, Sun QQ, Teh SK, Zhang W, Wen ZL, and Qu JY. (2013) Label-free in vivo imaging of human leukocytes using two-photon excited endogenous fluorescence. Journal of Biomedical Optics 18:040504. Li Y, Du XF, Wen ZL, and Du JL. (2012) Reciprocal regulation between resting microglial dynamics and neuronal activity in vivo. Developmental Cell 23:1189-1202. Li XL, Lan YH, Xu J, Zhang WQ, and Wen ZL. (2012) SUMO1-activating enzyme subunit 1 is essential for the survival of hematopoietic stem/progenitor cells in zebrafish. Development 139:4321-4329. Lu Q, Ye F, Wei Z, Wen ZL, and Zhang MJ. (2012) Antiparallel coiled-coil-mediated dimerization of myosin X. Proceedings of the National Academy of Sciences USA 109:17388-17393. Xu J, Du L, and Wen ZL. (2012) Myelopoiesis during zebrafish early development. Journal of Genetics and Genomics 39:435-442. (Invited review) Li L, Yan B, Shi YQ, Zhang WQ, and Wen ZL. (2012) Live imaging reveals differing roles of macrophages and neutrophils during zebrafish tail fin regeneration. The Journal of Biological Chemistry 287:25353-25360. Zeng Y, Xu J, Li D, Li L, Wen ZL, and Qu JY. (2012) Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging. Optics Letters 37:2490-2492. Jin H, Li L, Xu J, Zhen FH, Zhu L, Liu PP, Zhang MJ, Zhang WQ, and Wen ZL. (2012). Runx1 regulates embryonic myeloid fate choice in zebrafish through a negative feedback loop inhibiting Pu.1 expression. Blood 119:5239-5249. Zhang YY, Jin H, Li L, Qin X-F, and Wen ZL. (2011) cMyb regulates hematopoietic stem/progenitor cell mobilization during zebrafish hematopoiesis. Blood 118:4093-4101. Du LS, Xu J, Li XL, Ma N, Liu YM, Peng JR, Osato M, Zhang WQ, and Wen ZL. (2011) Rumba and Haus3 are essential factors for the maintenance of hematopoietic stem/progenitor cells during zebrafish hematopoiesis. Development 138:619-629. Li L, Jin H, Xu J, Shi YQ, and Wen ZL. (2011) Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis. Blood 117:1359-1369. 2006~2010 Ng CEL, Yokomizo T, Yamashita N, Cirovic B, Jin H, Wen ZL, Ito Y, and Osato MA. (2010) A Runx1 intronic enhancer marks hemogenic endothelial cells and hematopoietic stem cells. Stem Cells 28:1869-1881. Li D, Zhang YX, Xu L, Zhou LK, Wang Y, Xue BF, Wen ZL, Li P, and Sang JL. (2010) Regulation of gene expression by FSP27 in white and brown adipose tissue. BMC Genomics 11:446. Sood R,English MA, Belele CL, Jin H, Bishop K, Haskins R, McKinney MC, Chahal J, Weinstein BM, Wen ZL, and Liu PP. (2010) Development of multilineage adult hematopoiesis in the zebrafish with a Runx1 truncation mutation. Blood 115:2806-2809. Wang R, Wei Z, Jin H, Wu H, Yu C, Wen W, Chan L-N, Wen ZL, and Zhang M. (2009) Autoinhibition of UNC5b revealed by the cytoplasmic domain structure of the receptor. Molecular Cell 33:692-703. Jin H, Sood R, Xu J, Zhen FH, Liu PP, and Wen ZL. (2009) Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI. Development 136:647-654. Huang HH, Ruan H, Aw YM, Hussain A, Guo L, Gao C, Qian F, Leung T, Song H, Kimelman D, Wen ZL*, and Peng JR*. (2008) Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis. Development 135:3209-3218. (Co-corresponding author) Toh SY, Gong JY, Du GL, Li Z, Yang SQ, Ye J, Yao HL, Zhang YX, Xue BF, Li Q, Yang HY, Wen ZL, and Li P. (2008) Up-regulation of mitochondrial activity and acquirement of brown adipose tissue-like property in the white adipose tissue of Fsp27 deficient mice. PLoS ONE 3:e2890. Li JZ, Ye J, Xue BF, Qi JZ, Zhang J, Zhou ZH, Li Q, Wen ZL, and Li P. (2007) Cideb regulates diet-induced obesity, liver steatosis, and insulin sensitivity by controlling lipogenesis and fatty acid oxidation. Diabetes 56:2523-2532. Liu YM, Du LS, Osato M, Teo EH, Qian F, Jin H, Zhen FH, Xu J, Guo L, Huang HH, Chen J, Geisler R, Jiang YJ, Peng JR, and Wen ZL. (2007) The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development. Blood 110:99-106. Jin H, Xu J, and Wen ZL. (2007). Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development. Blood 109:5209-5214. Qian F, Zhen FH, Xu J, Huang M, Li WY, and Wen ZL. (2007) Distinct functions for different scl isoforms in zebrafish primitive and definitive hematopoiesis. PLoS Biology5:1110-1119. Huang H, Lu FI, Jia S, Meng S, Cao Y, Wang Y, Ma W, Yin K, Wen ZL, Thisse C, Thisse B, and Meng A. (2007) Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation. Development 134:979-88. Jin H, Xu J, Qian F, Du LS, Tan CY, Lin ZX, Peng JR, and Wen ZL. (2006) The 5′ zebrafish scl promoter targets transcription to the brain, spinal cord, and hematopoietic and endothelial progenitors. Developmental Dynamics 235:60-67. 2001~2005 Chen J, Ruan H, Ng SM, Gao C, Soo HM, Wu W, Zhang ZH, Wen ZL, Lane DP, and Peng JR. (2005) Loss of function of def selectively up-regulates Δ113p53 expression to arrest expansion growth of digestive organs in zebrafish. Genes & Development 19:2900-2911. Hussain A, Cao DN, Cheng H, Wen ZL, and Peng JR. (2005) Identification of the conserved serine/threonine residues important for gibberellin-sensitivity of Arabidopsis RGL2 protein. Plant Journal 44:88-99. Qian F, Zhen FH, Ong CT, Jin S-W, Soo HM, Stainier DYR, Lin S, Peng JR and Wen ZL. (2005) Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes. Developmental Dynamics 233:1163-1172. Wen CM, Zhang ZH, Ma WP, Xu M, Wen ZL, and Peng JR. (2004) Genome-wide identification of female-enriched genes in zebrafish. Developmental Dynamics 232:171-179. Lo J, Lee S, Xu M, Liu F, Ruan H, Eun A, He Y, Wang W, Wen ZL*, and Peng JR*. (2003) 15,000 unique zebrafish EST clusters and their future use in microarray for profiling gene expression patterns during embryogenesis. Genome Research 13:455-466. (Co-corresponding author) Shen S, Wen ZL, and Liu DX. (2003) Emergence of a coronavirus infectious bronchitis virus mutant with a truncated 3b gene: functional characterization of the 3b protein in pathogenesis and replication. Virology 311:16-27. Huang M, Qian F, Hu Y, Ang C, Li Z, and Wen ZL. (2002) Chromatin-remodelling factor BRG1 selectively activates a subset of interferon-alpha-inducible genes. Nature Cell Biology 4:774-781. Lam SH, Chua HL, Gong Z, Wen ZL, Lam TJ, and Sin YM. (2002) Morphologic transformation of the thymus in developing zebrafish. Developmental Dynamics 225:87-94. Liu F and Wen ZL. (2002) Cloning and expression pattern of the lysozyme C gene in zebrafish. Mechanisms of Development 113:69-72. ~2000 Yang E, Wen ZL, Haspel RL, and Darnell JE. (1999) The linker domain of Stat1 is required for gamma interferon-driven transcription. Molecular and Cellular Biology 19:5106-5112. Zhu X, Wen ZL, Xu L, and Darnell JE. (1997) Stat1 serine phosphorylation occurs independently of tyrosine phosphorylation and requires an activated Jak2 kinase. Molecular and Cellular Biology 17:6618-6623. Wen ZL and Darnell JE. (1997) Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3. Nucleic Acids Research 25:2062-2067. Duncan SA, Zhong Z, Wen ZL, and Darnell JE. (1997) STAT signaling is active during early mammalian development. Developmental Dynamics 208:190-198. Bromberg JF, Horvath CM, Wen ZL, Schreiber RD, and Darnell JE. (1996) Transcriptionally active Stat1 is required for the antiproliferative effects of both interferon α and interferon γ. Proceedings of the National Academy of Sciences USA 93:7673-7678. Wen ZL, Zhong Z, and Darnell JE. (1995) Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Cell 82:241-250. Horvath CM, Wen ZL, and Darnell JE. (1995) A STAT protein domain that determines DNA sequence recognition suggests a novel DNA-binding domain. Genes & Development 9:984-994. Boulton TG, Zhong Z, Wen ZL, Darnell JE, Stahl N, and Yancopoulos GD. (1995) Stat3 activation by cytokines utilizing gp130 and related transducers involves a secondary modification requiring an H7-sensitive kinase. Proceedings of the National Academy of Sciences USA 92:6915-6919. Gronowski AM, Zhong Z, Wen ZL, Thomas MJ, Darnell JE, and Rotwein P. (1995) In vivo growth hormone treatment rapidly stimulates the tyrosine phosphorylation and activation of Stat3. Molecular Endocrinology 9:171-177. Copeland NG, Gilbert DJ, Schindler C, Zhong Z, Wen ZL, Darnell JE, Mui AL-F, Quelle FW, Ihle JN, and Jenkins NA. (1995) Distribution of the mammalian Stat gene family in mouse chromosomes. Genomics 29:225-228. Yan RQ, Qureshi S, Zhong Z, Wen ZL, and Darnell JE. (1995) The genomic structure of the STAT genes-multiple exons in coincident sites in STAT1 and STAT2. Nucleic Acids Research 23:459-463. Zhong Z*, Wen ZL*, and Darnell JE. (1994) Stat3 and Stat4: members of the family of signal transducers and activators of transcription. Proceedings of the National Academy of Sciences USA 91:4806-4810. (*Co-first author) Ruff-Jamison S, Zhong Z, Wen ZL, Chen K, Darnell JE, and Cohen S. (1994). Epidermal growth factor and lipopolysaccharide activate Stat3 transcription factor in mouse liver. The Journal of Biological Chemistry 269:21933-21935. Zhong Z, Wen ZL, and Darnell JE. (1994) Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science 264:95-98. Solheim J, Carreno BM, Smith JD, Gorka J, Myers NB, Wen ZL, Martinko JM, Lee DR, and Hansen TH. (1993) Binding of peptides lacking consensus anchor residue alters H-2Ld serologic recognition. The Journal of Immunology 151:5387-5397. Tatro JB, Wen ZL, Entwistle ML, Atkins MB, Smith TJ, Reichlin S, and Murphy JR. (1992) Interaction of an α-melanocyte-stimulating hormone-diphtheria toxin fusion protein with melanotropin receptors in human melanoma metastases. Cancer Research 52:2545-2548. Lakkis F, Landgraf B, Wen ZL, Strom TB, and Murphy JR. (1992) Phe496 and Leu497 are essential for receptor binding and cytotoxic action of the murine interleukin-4 receptor targeted fusion toxin DAB389-mIL-4. Protein Engineering 5:241-248. Wen ZL, Tao X, Lakkis F, Kiyokawa T, and Murphy JR. (1991) Diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion toxin. Internal in-frame deletion from Thr387 to His485 results in the formation of a highly potent fusion toxin which is resistant to proteolytic degradation. The Journal of Biological Chemistry 266:12289-12293. Williams DP, Wen ZL, Watson SR, Boyd J, Strom TB, and Murphy JR. (1990) Cellular processing of the interleukin-2 fusion toxin DAB486-IL-2 and efficient delivery of diphtheria fragment A to the cytosol of target cells requires Arg194. The Journal of Biological Chemistry 265:20673-20677.